UNC-30/PITX coordinates neurotransmitter identity with postsynaptic GABA receptor clustering.

Terminal selectors are transcription factors that control neuronal identity by regulating the expression of key effector molecules, such as neurotransmitter (NT) biosynthesis proteins, ion channels and neuropeptides. Whether and how terminal selectors control neuronal connectivity is poorly understood. Here, we report that UNC-30 (PITX2/3), the terminal selector of GABA motor neuron identity in C. elegans , is required for NT receptor clustering, a hallmark of postsynaptic differentiation. Animals lacking unc-30 or madd-4B, the short isoform of the MN-secreted synapse organizer madd-4 ( Punctin/ADAMTSL ), display severe GABA receptor type A (GABA A R) clustering defects in postsynaptic muscle cells. Mechanistically, UNC-30 acts directly to induce and maintain transcription of madd-4B and GABA biosynthesis genes (e.g., unc-25/GAD , unc-47/VGAT ). Hence, UNC-30 controls GABA A R clustering on postsynaptic muscle cells and GABA biosynthesis in presynaptic cells, transcriptionally coordinating two critical processes for GABA neurotransmission. Further, we uncover multiple target genes and a dual role for UNC-30 both as an activator and repressor of gene transcription. Our findings on UNC-30 function may contribute to our molecular understanding of human conditions, such as Axenfeld-Rieger syndrome, caused by PITX2 and PITX3 gene mutations.

Data on inheritance of race 2 anthracnose resistance in watermelon (Citrullus spp.) biparental mapping populations.

Anthracnose of watermelon is caused by a fungal pathogen Colletotrichum orbiculare. We generated F2 individuals from three different populations: Population 1 (PI 189225 x 'New Hampshire Midget'), Population 2 ('Perola' x PI 189225), and Population 3 ('Verona' x PI 189225). The biparental F2 populations, parents and F1 individuals were inoculated with an isolate of race 2 anthracnose isolated from watermelon. Leaf lesions were visually rated seven days post inoculation on a scale of 0% (no lesion) to 100% (dead true leaf). Here we present the datasets obtained after the disease inoculation. The distribution of data obtained was visualized using histograms and goodness-of-fit was tested using Chi-Square. These datasets provide information on the mode of inheritance of race 2 anthracnose resistance in watermelon.

Novel Feature-Extraction Methods for the Estimation of Above-Ground Biomass in Rice Crops.

Traditional methods to measure spatio-temporal variations in above-ground biomass dynamics (AGBD) predominantly rely on the extraction of several vegetation-index features highly associated with AGBD variations through the phenological crop cycle. This work presents a comprehensive comparison between two different approaches for feature extraction for non-destructive biomass estimation using aerial multispectral imagery. The first method is called GFKuts, an approach that optimally labels the plot canopy based on a Gaussian mixture model, a Montecarlo-based K-means, and a guided image filtering for the extraction of canopy vegetation indices associated with biomass yield. The second method is based on a Graph-Based Data Fusion (GBF) approach that does not depend on calculating vegetation-index image reflectances. Both methods are experimentally tested and compared through rice growth stages: vegetative, reproductive, and ripening. Biomass estimation correlations are calculated and compared against an assembled ground-truth biomass measurements taken by destructive sampling. The proposed GBF-Sm-Bs approach outperformed competing methods by obtaining biomass estimation correlation of 0.995 with R2=0.991 and RMSE=45.358 g. This result increases the precision in the biomass estimation by around 62.43% compared to previous works.

Coronavirus disease 2019 in Latin American patients with multiple sclerosis.

Patients with multiple sclerosis (MS) who present coronavirus disease 2019 (COVID-19) are of particular interest to neurologists. These patients have a neuroimmune disease and receive immunomodulatory or immunosuppressive therapies in the long-term. We present here data from 73 patients with MS and a confirmed diagnosis of COVID-19 from five Latin American countries. Fifteen patients (20.5%) were hospitalized and two patients died. The use of anti-CD20 therapies was the only risk factor associated to hospitalization and death. Despite the small sample size, this study highlights the awareness regarding therapeutic options for MS during the pandemic.

A novel NIR-image segmentation method for the precise estimation of above-ground biomass in rice crops.

Traditional methods to measure spatio-temporal variations in biomass rely on a labor-intensive destructive sampling of the crop. In this paper, we present a high-throughput phenotyping approach for the estimation of Above-Ground Biomass Dynamics (AGBD) using an unmanned aerial system. Multispectral imagery was acquired and processed by using the proposed segmentation method called GFKuts, that optimally labels the plot canopy based on a Gaussian mixture model, a Montecarlo based K-means, and a guided image filtering. Accurate plot segmentation results enabled the extraction of several canopy features associated with biomass yield. Machine learning algorithms were trained to estimate the AGBD according to the growth stages of the crop and the physiological response of two rice genotypes under lowland and upland production systems. Results report AGBD estimation correlations with an average of r = 0.95 and R2 = 0.91 according to the experimental data. We compared our segmentation method against a traditional technique based on clustering. A comprehensive improvement of 13% in the biomass correlation was obtained thanks to the segmentation method proposed herein.

Establishment and maintenance of motor neuron identity via temporal modularity in terminal selector function.

Terminal selectors are transcription factors (TFs) that establish during development and maintain throughout life post-mitotic neuronal identity. We previously showed that UNC-3/Ebf, the terminal selector of C. elegans cholinergic motor neurons (MNs), acts indirectly to prevent alternative neuronal identities (Feng et al., 2020). Here, we globally identify the direct targets of UNC-3. Unexpectedly, we find that the suite of UNC-3 targets in MNs is modified across different life stages, revealing 'temporal modularity' in terminal selector function. In all larval and adult stages examined, UNC-3 is required for continuous expression of various protein classes (e.g. receptors, transporters) critical for MN function. However, only in late larvae and adults, UNC-3 is required to maintain expression of MN-specific TFs. Minimal disruption of UNC-3's temporal modularity via genome engineering affects locomotion. Another C. elegans terminal selector (UNC-30/Pitx) also exhibits temporal modularity, supporting the potential generality of this mechanism for the control of neuronal identity.

An ancient role for collier/Olf/Ebf (COE)-type transcription factors in axial motor neuron development.

BACKGROUND: Mammalian motor circuits display remarkable cellular diversity with hundreds of motor neuron (MN) subtypes innervating hundreds of different muscles. Extensive research on limb muscle-innervating MNs has begun to elucidate the genetic programs that control animal locomotion. In striking contrast, the molecular mechanisms underlying the development of axial muscle-innervating MNs, which control breathing and spinal alignment, are poorly studied. METHODS: Our previous studies indicated that the function of the Collier/Olf/Ebf (COE) family of transcription factors (TFs) in axial MN development may be conserved from nematodes to simple chordates. Here, we examine the expression pattern of all four mouse COE family members (mEbf1-mEbf4) in spinal MNs and employ genetic approaches in both nematodes and mice to investigate their function in axial MN development. RESULTS: We report that mEbf1 and mEbf2 are expressed in distinct MN clusters (termed "columns") that innervate different axial muscles. Mouse Ebf1 is expressed in MNs of the hypaxial motor column (HMC), which is necessary for breathing, while mEbf2 is expressed in MNs of the medial motor column (MMC) that control spinal alignment. Our characterization of Ebf2 knock-out mice uncovered a requirement for Ebf2 in the differentiation program of a subset of MMC MNs and revealed for the first time molecular diversity within MMC neurons. Intriguingly, transgenic expression of mEbf1 or mEbf2 can rescue axial MN differentiation and locomotory defects in nematodes (Caenorhabditis elegans) lacking unc-3, the sole C. elegans ortholog of the COE family, suggesting functional conservation among mEbf1, mEbf2 and nematode UNC-3. CONCLUSIONS: These findings support the hypothesis that genetic programs controlling axial MN development are deeply conserved across species, and further advance our understanding of such programs by revealing an essential role for Ebf2 in mouse axial MNs. Because human mutations in COE orthologs lead to neurodevelopmental disorders characterized by motor developmental delay, our findings may advance our understanding of these human conditions.

Prevalence of multiple sclerosis in Latin America and its relationship with European migration.

Multiple sclerosis (MS) is a chronic, degenerative autoimmune inflammatory disease of the central nervous system. The prevalence is different in every continent, changing according to geographical and environmental characteristics. The areas with the highest prevalence in the world are Europe and North America. In Latin America, the prevalence is higher in areas where there was greater European migration, as in the case of Argentina, Chile, Brazil, Uruguay and Mexico, and there have been no identified cases amongst native Indian populations. It should be considered that environmental factors may influence the prevalence of MS in Latin America, and it seems as if there are protective factors such as exposure to ultraviolet radiation and the presence of parasitosis.

Unusually Located Stroke After Chemotherapy in Testicular Germ Cell Tumors.

Testicular cancer is a type of malignancy that affects young adults and has high rates of cure; however, as any malignancy, it is associated with an increased risk of ischemic or hemorrhagic cerebrovascular disease, given the systemic tumor effects or side effects of chemotherapy, which in turn increases morbidity, functional impairment, and additional risk of early death.

Traumatic dissection of the internal carotid artery: simultaneous infarct of optic nerve and brain.

KEY CLINICAL MESSAGE: Traumatic intracranial internal carotid artery dissection is a rare but significant cause of stroke in patients in their forties, leading to high morbidity and mortality. Simultaneous ischemic stroke and optic nerve infarction can occur. Clinical suspicion of dissection is determining in the acute management.

HLA Alleles and Dengue Virus Infection in Venezuelan Patients: A Preliminary Study

En áreas endémicas, una pequeña proporción de individuos infectados con el virus dengue desarrollan la fiebre hemorrágica del dengue (FHD),sugiriendo la existencia de factores de resistencia que pudiesen estar jugando un papel importante en el huésped. Este trabajo describe la frecuencia dealelos HLA clase I y II en pacientes con dengue y su relación con las manifestaciones clínicas de la enfermedad. El análisis de la frecuencia de especificidades HLA en los pacientes con dengue mostró frecuencias disminuidas de los alelos B*15, B*49, DRB1*02 y DRB1*03 y frecuencias incrementadas de los alelos B*57 y DRB1*15 comparado con los controles. Cuando lospacientes fueron agrupados y comparados de acuerdo a la severidad de laenfermedad, se observó una susceptibilidad incrementada a la fiebre del dengue clásico (FD) en pacientes con B*57, una susceptibilidad disminuida aldesarrollo de la FHD en pacientes A*03 y una susceptibilidad incrementada al desarrollo de la FHD en pacientes con B*40. Aunque las asociacionesobservadas proceden de un estudio poblacional caso-control relativamentepequeño y que después de la corrección por múltiples comparaciones se mantuvo únicamente la asociación con DRB1*15, los datos confirman que posiblemente los alelos HLA pueden jugar un papel en la susceptibilidad y/oresistencia a la infección por virus dengue y al desarrollo de la FHD (AU)

In endemic areas, a small proportion of individuals infected with dengue virus develop dengue hemorrhagic fever (DHF) suggesting that theremay be host specific resistance factors playing an important role. Thiswork describes the frequency of HLA class I and class II alleles in patientswith dengue and the relationship with the clinical manifestations of thedisease. The analysis of the frequency of HLA specificities in the dengue patients revealed reduced frequencies of B*15, B*49, DRB1*02 andDRB1*03 and increased frequencies of B*57 and DRB1*15 compared withcontrols. When the patients were grouped and compared according todisease severity, an association with enhanced susceptibility to denguefever (DF) in patients with B*57, an association with reduced susceptibility to DHF in patients with A*03, and an association with enhanced susceptibility to DHF in patients with B*40 was observed. Although the associations revealed in this study come from a very small case-control population and that after correction for multiple testing only the associationwith DRB1*15 is maintained, the data suggest that the HLA alleles canpossibly play a role in the susceptibility and/or resistance to dengue virusinfection and development of DHF (AU)